Intended use: Test for the quantitative determination of the total activity of α?amylase (EC 188.8.131.52) in blood, serum or
plasma with Reflotron systems.
The α?amylase (1,4?α,D?glycanohydrolase, EC 184.108.40.206) catalyzes the hydrolytic breakdown of polymeric carbohydrates like
amylose, amylopectin and glycogen by cleavage of 1,4?α?glucosidic bonds. In poly- and oligosaccharides, several
glucosidic bonds are simultaneously hydrolyzed. Being the smallest unit, maltotriose is cleaved into maltose and glucose, at
a much slower rate.
Two types of α?amylase are distinguished: the pancreatic type (P?type) and the salivary gland type (S?type). The P?type
comes almost exclusively from the pancreas, and is thus organ-specific; the S?type can have various sources.
Apart from in the salivary glands, it can also occur in tears, sweat, breast milk, amniotic fluid, lungs, testes and the
epithelium of the oviducts. On account of the rather non-specific clinical symptoms of pancreatic disease, enzyme
determinations are of particular value in the diagnosis of these disorders. They are performed particularly for the diagnosis
and monitoring of acute pancreatitis. However, hyperamylasaemia does not only occur in acute pancreatitis, or in the
inflammatory phase of chronic pancreatitis but also in renal failure as a result of reduced glomerular filtration, in tumours of
the lung, or the ovaries, in disorders of the salivary glands, diabetic ketoacidosis, cerebral trauma, after surgery or in
macroamylasemia or in pneumonia. To confirm the pancreatic origin, additional determination of a further pancreas-specific
enzyme like pancreatic α?amylase is recommended.
Available in a tube of 15 test/strips.